CHEMILUMINESCENT REAGENTS FOR WESTERN BLOTTING

Supplemental Product Information:

Product Information

• Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
• Volume : 1 kit (for 2000 cm² of membrane)

Description

• This product is a reagent for the chemiluminescent detection of HRP-labeled probes that react with proteins transferred to the membrane by Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. It allows the detection of proteins at the nanogram level.

Storage

• Store at 4 °C, protected from light. Use within 6 months.

Direction for Use

• 1. Bring Solution A and Solution B to room temperature.
  2. Prepare a blotting membrane covered with HRP-conjugated probe.
  3. Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
  4. Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm² membrane.
  5. Place the membrane with the protein blotted side up on the plastic wrap or polyethylene sheet.
  6. Pour the mixture over the surface of the membrane.
  7. Incubate the membrane with this product for 60 seconds at room temperature.
  8. Remove excess mixture using paper.
  9. Place the membrane in a new wrap or polyethylene sheet.
  10. Image the membrane using an imaging system or X-ray film.