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The microscope objective will be engraved OIL, OEL, or HI if the objective is designed to function with immersion oil. If not, the objective is meant to be used dry. Remember to clean the immersion oil objective after use.
Microscope objectives are intended to image specimens either through air or a medium of higher refractive index between the front lens and the specimen. A gain in resolution by a factor of about 1.5 is attained when immersion oil is substituted for air as the imaging medium.
The advantages of oil immersion objectives are severely compromised if the wrong immersion fluid is utilized. Microscope manufacturers produce objectives with tight tolerances to refractive index and dispersion, which require matching values in the liquid placed between the cover glass and objective front lens. It is advisable to employ only the oil intended by the objective manufacturer, and to not mix immersion oils between manufacturers to avoid unpleasant artifacts such as crystallization or phase separation.
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