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USP L109: Spherical particles of porous graphitic carbon, 3 to 30 μm in diameter

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Hypercarb™ has unique properties as a packing for HPLC, which differ from those of silica-based stationary phases. It has exceptional ability to retain very polar compounds and to separate closely related compounds. The hydrophobic and electrostatic retention mechanisms that explain this behavior also require a different approach to develop a method on this stationary phase. In this guide we have reviewed a successful approach to method development on Hypercarb columns.

Method Development Guide for Hypercarb columns

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