Aeris Core-Shell LC Columns for Proteins and Peptides

Aeris is a range of high-performance reversed-phase liquid chromatography columns specifically developed for the separation of proteins, peptides, and complex biomolecular mixtures. Based on Core-Shell particle technology, these columns provide high separation efficiency, sharp peak shapes, and low backpressure.

The unique particle structure shortens the diffusion path of molecules within the stationary phase, helping to improve resolution and separation efficiency. Aeris columns deliver high-performance chromatographic results on conventional HPLC and modern UHPLC systems, expanding method optimization capabilities without necessarily requiring equipment capable of ultra-high operating pressures.

The product range includes two main series: Aeris WIDEPORE, designed for intact proteins and large polypeptides, and Aeris PEPTIDE, developed for peptide separations and peptide mapping.

Key Advantages of Aeris

• High resolution — effective separation of complex protein and peptide mixtures, including components with similar chromatographic properties.

• Core-Shell technology — a solid core surrounded by a thin porous shell shortens analyte diffusion paths and reduces chromatographic peak broadening.

• Low backpressure — 3.6 µm particles provide high separation efficiency at moderate backpressure, allowing the columns to be used on conventional HPLC systems.

• High analytical sensitivity — narrow peaks support better separation of closely eluting components and improve the quality of analytical results.

• Reduced irreversible protein adsorption — specialized surface chemistry promotes higher protein recovery and more reproducible results.

• Low stationary-phase bleed — helps reduce background signals and interference during LC-MS analysis.

• High chemical and temperature stability — a pH operating range of 1.5–9 and operating temperatures up to 90 °C expand method development and optimization capabilities.

• Reproducible performance — controlled particle characteristics, porosity, and sorbent surface properties help ensure consistent column performance between batches.

• Flexible column selection — different surface chemistries, particle sizes, and column lengths allow optimization of selectivity, resolution, and analysis time.

  • XB-C18 — provides maximum hydrophobic retention and is suitable for very hydrophobic proteins and membrane proteins.

  • XB-C8 — provides moderate hydrophobic retention and is suitable for monoclonal antibodies and glycosylated proteins.

  • C4 — provides lower hydrophobic retention and is suitable for hydrophilic proteins, PEGylated proteins, and challenging protein separations.