Onyx Monolithic LC Columns

Onyx is a range of monolithic silica HPLC columns from Phenomenex, designed for high-speed analysis, increased laboratory throughput, and efficient separation of complex samples.

Unlike conventional particle-packed columns, Onyx columns are manufactured as a porous monolithic silica rod. Their highly porous structure provides efficient mobile-phase flow with reduced flow resistance, allowing high flow rates without excessive backpressure.

Key Benefits of Onyx

• Significantly reduced analysis time — methods can be shortened by more than half, with some applications achieving analysis times of less than 4 minutes.
• High flow rates enabled by the highly porous monolithic structure.
• Low backpressure — reduces stress on pumps, seals, and the HPLC system.
• Increased sample throughput — analyze more samples in less time.
• Efficient handling of complex and viscous samples — the macroporous structure allows mobile phase and samples to flow through the column with less restriction compared with particle-packed columns.
• “Dilute-and-shoot” capability — minimizes sample preparation and saves valuable laboratory time.
• Reduced risk of flow restrictions and overpressure caused by salts, precipitated proteins, and lipids in the sample matrix.
• Reduced sample carryover when analyzing complex matrices such as urine and plasma.
• No inlet bed settling — provides increased reliability, reproducibility, and column lifetime.
• Enhanced resolution of complex mixtures by coupling columns in series.